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Now that single-molecule detection was no longer necessary, Solexa was able to develop its signature sequencing chemistry. The process takes place on a chip called a flow cell, which contains a lawn of short DNA sequences affixed to its surface. The template DNA is broken up into small fragments, and adapter sequences, complementary to the DNA on the flow cell’s surface, are added to the ends of each fragment. DNA fragments are then passed over the flow cell, where the adapter sequences bind to spots on the DNA lawn. At this point, primers are added, and an initial round of amplification takes place: the short DNA sequences on the flow cell are extended to create sequences complementary to the bound template DNA fragments, which are then washed away. The sequences present in the fragments of template DNA are now affixed directly to the flow cell.
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第五十九条 就海上货物运输合同所涉及的货物灭失、损坏或者迟延交付通过诉讼、仲裁等方式对承运人提起的任何赔偿请求，无论海事请求人是否为合同的一方，也无论是根据合同或者侵权行为提出请求，均适用本章关于承运人的抗辩理由和限制赔偿责任的规定。。谷歌浏览器下载对此有专业解读
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